| Expression pattern: |
UP |
| Associated gene: |
IL6R, JAK1, STAT3, cyclin D1, Bcl-2, Bax |
| Associated microRNA: |
miR-449a |
| Biological function: |
Promotes OS cell proliferation and cell cycle progression, inhibits apoptosis, and contributes to carcinogenesis of OS. |
| Molecular mechanism: |
circ_0009910 functions as a miR-449a sponge, upregulates the miR-449a target IL6R, and activates downstream JAK1/STAT3 signaling. |
| Biological pathway or process: |
ceRNA regulation (promotes); JAK/STAT (promotes); proliferation (promotes); cell cycle (promotes); apoptosis (inhibits) |
| Detected method: |
Q
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RT-qPCR; Clinical Sample Validation; Bioinformatics Analysis; Transfection; MTT; Cell Cycle Assay; Annexin V/PI Flow Cytometry; Luciferase Reporter Assay; Western Blot |
| Clinical significance: |
- |
| Description: |
hsa_circ_0009910 is up-regulated in osteosarcoma cells and tissues. It promotes OS cell proliferation and cell cycle progression while suppressing apoptosis by sponging miR-449a, thereby increasing IL6R expression and activating JAK1/STAT3 signaling. |
| Confidence score: |
0.7164 |