| Expression pattern: |
UP |
| Associated gene: |
SOX4 |
| Associated microRNA: |
miR-338-3p |
| Biological function: |
Promotes CAF migration and invasion; enhances A549 cell proliferation, migration and invasion; promotes NSCLC tumour growth in vivo. |
| Molecular mechanism: |
circFARP1 acts as a miR-338-3p sponge in CAFs and antagonizes miR-338-3p-mediated inhibition of SOX4, thereby regulating SOX4 expression and secretion to promote NSCLC progression. |
| Biological pathway or process: |
ceRNA regulation (promotes); proliferation (promotes); migration (promotes); invasion (promotes); metastasis (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; FISH / smFISH; RNA Pull-Down; Luciferase Reporter Assay; Transfection; CCK8; Transwell Assay; Western Blot; RIP (RNA Immunoprecipitation); IHC (Immunohistochemistry); In Vivo Animal Model; Clinical Sample Validation; Bioinformatics Analysis |
| Clinical significance: |
circFARP1 might be a potential therapy target for NSCLC. |
| Description: |
circFARP1 is up-regulated in cancer-associated fibroblasts from NSCLC and promotes CAF migration/invasion as well as A549 proliferation, migration, invasion and tumor growth. Mechanistically, circFARP1 functions as a miR-338-3p sponge to relieve inhibition of SOX4, forming a circFARP1/miR-338-3p/SOX4 axis that supports NSCLC progression and may represent a therapeutic target. |
| Confidence score: |
0.7932 |