| Expression pattern: |
UP |
| Associated gene: |
- |
| Associated microRNA: |
hsa-mir-448, hsa-mir-150, hsa-mir-93 |
| Biological function: |
promotes proliferation and invasion, promotes tumor growth, inhibits apoptosis |
| Molecular mechanism: |
acts as a miRNA sponge for miR-448 |
| Biological pathway or process: |
proliferation (promotes); invasion (promotes); apoptosis (inhibits); metastasis (promotes); ceRNA regulation (other) |
| Detected method: |
Q
M
|
| Validation methods: |
Microarray; RNase R Treatment; RT-qPCR; Back-Splice Junction PCR / divergent primers PCR; Clinical Sample Validation; ROC Analysis; Luciferase Reporter Assay; Transfection; CCK8; Colony Formation Assay; Annexin V/PI Flow Cytometry; Transwell Assay; In Vivo Animal Model |
| Clinical significance: |
circ-NT5C2 could act as a valuable diagnostic marker for osteosarcoma detection with AUC (area under the ROC curve) value of 0.753. |
| Description: |
circ-NT5C2 is up-regulated in osteosarcoma tissues/cell lines and functions as an oncogenic circRNA. Knockdown inhibits proliferation and invasion and promotes apoptosis, and suppresses xenograft tumor growth. Mechanistically, circ-NT5C2 acts as a sponge for miR-448 and shows negative correlation with miR-448. |
| Confidence score: |
0.8034 |