| Expression pattern: |
DN |
| Associated gene: |
AGO2 |
| Associated microRNA: |
miR-638 |
| Biological function: |
Suppresses leukemia cell proliferation and promotes ferroptosis; overexpression reduces EdU-positive leukemia cells, suppresses proliferation, sensitizes cells to RSL3-induced ferroptosis, and counteracts Fer-1-induced inhibition of ferroptosis. |
| Molecular mechanism: |
circTADA2A functions as a cytoplasmic miRNA sponge for miR-638, sequestering miR-638 and inhibiting its activity; miR-638 overexpression rescues circTADA2A-mediated inhibition of proliferation and promotion of ferroptosis. |
| Biological pathway or process: |
proliferation (inhibits); ferroptosis (promotes); ceRNA regulation (other) |
| Detected method: |
Q
M
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; Actinomycin D / DRB Stability Assay; RT-qPCR; Microarray; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; Transfection; CCK8; EdU Staining; Flow Cytometry（Non-apoptosis/cycle）; ROC Analysis; Bioinformatics Analysis |
| Clinical significance: |
Plasma circTADA2A may serve as a non-invasive diagnostic biomarker for AML and may distinguish AML cytogenetic risk groups; its expression is associated with bone marrow blasts and cytogenetic risk status. |
| Description: |
circTADA2A is a human circRNA derived from TADA2A and is downregulated in plasma, bone marrow, and AML cell lines. In AML models, circTADA2A suppresses leukemia cell proliferation and facilitates ferroptosis by sponging miR-638, while plasma circTADA2A shows potential diagnostic value and association with cytogenetic risk. |
| Confidence score: |
0.8794 |