circRNA basic information
circBase ID: -
Name: hsa_circ_DNMT1
Synonym: circ-DNMT1 / hsa_circRNA_102439
Host Gene: Dnmt1
Genomic location(hg19): -
Genomic location(hg38): -
Subcellular localization: nucleus and cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0007254
MONDO name: breast cancer
Disease details: breast cancer / BC
Disease DO ID:
 1612
Disease MeSH ID:
 -
Disease NCIt ID:
 C9335
Disease ICD11 ID:
 1047754165
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

tumor tissues; adjacent tissues
Cell lines:

MCF-7; MDA-MB-468; MDA-MB-231; HTB126; SK-BR-3; MCF-10A; HaCaT; 293T; BEAS2B; Jurkat; HUVEC; H460; LnCap; Du145; PC3; JHH1; SNU449; HepG2; Hela
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: p53, AUF1, Auf1, Dnmt1 mRNA, Dnmt1, LC3B
Associated microRNA: -
Biological function: Enhances breast cancer cell proliferation and survival, stimulates cellular autophagy, inhibits cellular senescence, and increases tumor xenograft growth.
Molecular mechanism: circ-Dnmt1 binds p53 and AUF1/Auf1, promotes their nuclear translocation, induces autophagy, stabilizes Dnmt1 mRNA and increases Dnmt1 translation, leading to inhibition of p53 transcription.
Biological pathway or process:

autophagy (promotes); proliferation (promotes); mRNA stability (promotes); other pathway/process (inhibits)
Detected method:
Q
M
Validation methods:

Microarray; RT-qPCR; Clinical Sample Validation; RNase R Treatment; Northern Blot; Back-Splice Junction PCR / divergent primers PCR; Sanger Sequencing; Transfection; Western Blot; IF (Immunofluorescence); Nuclear-Cytoplasmic Fractionation; RIP (RNA Immunoprecipitation); RNA Pull-Down; ISH (In Situ Hybridization); IHC (Immunohistochemistry); In Vivo Animal Model; Survival Analysis; Bioinformatics Analysis
Clinical significance:

circ-Dnmt1 is significantly upregulated in breast carcinoma patient tumors compared with adjacent tissues and is described as an oncogenic circular RNA with potential for further preclinical research.
Description:

circ-Dnmt1 is upregulated in breast cancer cell lines and breast carcinoma tumors. It functions as an oncogenic circRNA by binding p53 and AUF1/Auf1, promoting their nuclear translocation, enhancing autophagy, increasing Dnmt1 expression, inhibiting p53 transcription, reducing senescence, and promoting proliferation, survival, and xenograft tumor growth.
Confidence score:

0.8779
Other information
Title:

A circular RNA circ-DNMT1 enhances breast cancer progression by activating autophagy.

Journal: Oncogene
Published: 2018
PubMed ID: 29973691
Study type:

combined biological and clinical study
Data availability: -
Code availability: -