| Expression pattern: |
UP |
| Associated gene: |
HIF1alpha, PTBP1, pre-TDRD3, TDRD3 promoter |
| Associated microRNA: |
miR-1231 |
| Biological function: |
Promotes CRC cell proliferation, migration, invasion, tumor growth and metastasis. |
| Molecular mechanism: |
circTDRD3 acts as a ceRNA sponge for miR-1231 to upregulate HIF1alpha; HIF1alpha transcriptionally activates TDRD3 pre-mRNA and induces PTBP1, which promotes circTDRD3 biogenesis, forming a positive feedback loop. |
| Biological pathway or process: |
ceRNA regulation (promotes); proliferation (promotes); migration (promotes); invasion (promotes); metastasis (promotes); EMT (promotes); angiogenesis (promotes); HIF-1 signaling (promotes) |
| Detected method: |
Q
H
S
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; RT-qPCR; RNA-seq; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; RIP (RNA Immunoprecipitation); RNA Pull-Down; ChIP / ChIP-seq; Luciferase Reporter Assay; Transfection; CCK8; EdU Staining; Colony Formation Assay; Transwell Assay; Wound Healing Assay; IHC (Immunohistochemistry); Western Blot; In Vivo Animal Model; H&E Staining; Cohort Study; Survival Analysis; Bioinformatics Analysis |
| Clinical significance: |
High circTDRD3 expression correlates with larger tumor size, TNM stage, lymph node invasion, lower OS and DFS, and may serve as a prognostic biomarker and therapeutic target for CRC. |
| Description: |
circTDRD3 is a hypoxia-induced, cytoplasmic circRNA upregulated in CRC tissues and cell lines. It promotes CRC proliferation, invasion and metastasis by sponging miR-1231 to increase HIF1alpha, while HIF1alpha and PTBP1 further enhance circTDRD3 biogenesis, forming a tumor-promoting positive feedback loop. High circTDRD3 expression is associated with unfavorable clinicopathological features and poor survival. |
| Confidence score: |
0.8981 |