| Expression pattern: |
UP |
| Associated gene: |
STAT5A, AGO2 |
| Associated microRNA: |
miR-339-5p |
| Biological function: |
accelerates PCa proliferation, migration, invasion, tumor growth and progression |
| Molecular mechanism: |
circ_0086722 acts as a miR-339-5p sponge and relieves miR-339-5p-mediated repression of STAT5A, thereby elevating STAT5A to promote PCa progression |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); ceRNA regulation (promotes); other pathway/process (promotes) |
| Detected method: |
Q
M
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; Actinomycin D / DRB Stability Assay; RT-qPCR; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; Transfection; CCK8; Colony Formation Assay; Transwell Assay; In Vivo Animal Model; IHC (Immunohistochemistry); Western Blot; Cohort Study; Survival Analysis; Bioinformatics Analysis |
| Clinical significance: |
high levels of circ_0086722 were positively correlated with pT3 stage, higher specimen Gleason score (>7), and worse biochemical recurrence-free survivals; it may function as a potential prognostic biomarker or therapeutic target for PCa treatment |
| Description: |
circ_0086722 is an up-regulated UBAP2-derived circRNA in prostate cancer tissues and cell lines. It promotes PCa proliferation, migration, invasion and xenograft tumor growth by sponging miR-339-5p and increasing STAT5A expression. High circ_0086722 expression is associated with more advanced pathological features and worse biochemical recurrence-free survival, suggesting prognostic and therapeutic relevance. |
| Confidence score: |
0.8959 |