| Expression pattern: |
DN |
| Associated gene: |
SERP1 |
| Associated microRNA: |
miR-217-5p |
| Biological function: |
Protects against LPS-induced inflammatory and apoptotic injury, permeability enhancement and tubule formation arrest in HPMECs. |
| Molecular mechanism: |
circWDR33 functions through a ceRNA mechanism by targeting miR-217-5p and regulating SERP1. |
| Biological pathway or process: |
ceRNA regulation (promotes); inflammation (inhibits); apoptosis (inhibits); angiogenesis (promotes); other pathway/process (inhibits) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Actinomycin D / DRB Stability Assay; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; RNA Pull-Down; Luciferase Reporter Assay; Transfection; ELISA; Annexin V/PI Flow Cytometry; Transwell Assay; Tube Formation Assay; Western Blot; Bioinformatics Analysis |
| Clinical significance: |
A new potential therapeutic approach for sepsis-ALI patients. |
| Description: |
circWDR33 is down-regulated in sera from sepsis-ALI patients and in LPS-challenged HPMECs. Its overexpression protects HPMECs from LPS-induced inflammation, apoptosis, permeability increase, and tubule formation impairment through the miR-217-5p/SERP1 ceRNA axis, suggesting therapeutic potential in sepsis-ALI. |
| Confidence score: |
0.7795 |