| Expression pattern: |
DN |
| Associated gene: |
IFIT3 |
| Associated microRNA: |
hsa-miR-7150 |
| Biological function: |
Inhibits LUAD cell proliferation, promotes ferroptosis, and suppresses tumor growth. |
| Molecular mechanism: |
Acts as a miRNA sponge for hsa-miR-7150 to regulate IFIT3 expression; directly binds IFIT3 and may stabilize IFIT3 protein by affecting ubiquitination/protein stability. |
| Biological pathway or process: |
proliferation (inhibits); ferroptosis (promotes); immune regulation (other); ceRNA regulation (other); ubiquitination (other) |
| Detected method: |
Q
M
|
| Validation methods: |
Microarray; RT-qPCR; Back-Splice Junction PCR / divergent primers PCR; Sanger Sequencing; Clinical Sample Validation; Survival Analysis; ROC Analysis; Transfection; CCK8; IF (Immunofluorescence); Western Blot; RNA Pull-Down; RIP (RNA Immunoprecipitation); Co-IP; Bioinformatics Analysis; In Vivo Animal Model |
| Clinical significance: |
circ_BBS9 may serve as a diagnostic biomarker; low circ_BBS9 expression correlated with poor prognosis; patients with high circ_BBS9 expression had a better survival prognosis; ROC AUC=0.5693 for LUAD diagnosis. |
| Description: |
circ_BBS9 (has_circ_0003162), derived from the BBS9 gene, is down-regulated in LUAD tissues and cell lines and acts as a tumor suppressor. Its overexpression inhibits LUAD cell proliferation and promotes ferroptosis, and it mechanistically binds IFIT3 and participates in a circ_BBS9/hsa-miR-7150/IFIT3 regulatory axis. Clinically, higher circ_BBS9 expression is associated with better survival and shows diagnostic potential by ROC analysis. |
| Confidence score: |
0.8703 |