| Expression pattern: |
UP |
| Associated gene: |
PTBP1, ETV1, Ago2 |
| Associated microRNA: |
miR-195-5p |
| Biological function: |
Increases BTB tight junction-related proteins and decreases BTB permeability; its knockdown increases BTB permeability and enhances doxorubicin transport, promoting glioma cell apoptosis in the in vitro BTB model. |
| Molecular mechanism: |
PTBP1 promotes circRNA_001160 biogenesis; circRNA_001160 acts as a miR-195-5p sponge in RISC, relieving miR-195-5p repression of ETV1; ETV1 transcriptionally activates tight junction-related proteins (ZO-1, occludin, claudin-5) to reduce BTB permeability. |
| Biological pathway or process: |
ceRNA regulation (promotes); apoptosis (other) |
| Detected method: |
Q
B
H
M
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; RT-qPCR; Northern Blot; FISH / smFISH; Microarray; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; Transfection; Western Blot |
| Clinical significance: |
- |
| Description: |
circRNA_001160 (from ANKRD17) is up-regulated in glioma endothelial cells and decreases BTB permeability by maintaining tight-junction protein expression. Mechanistically, PTBP1 promotes its biogenesis, and circRNA_001160 sponges miR-195-5p to relieve repression of ETV1, which transcriptionally activates ZO-1/occludin/claudin-5. Targeting circRNA_001160 (e.g., knockdown) increases BTB permeability and can enhance doxorubicin passage in the in vitro BTB model. |
| Confidence score: |
0.7531 |