| Expression pattern: |
UP |
| Associated gene: |
CYP24A1 |
| Associated microRNA: |
miR-3611 |
| Biological function: |
circ_0005962 promotes LUAD cell proliferation, cell cycle progression, colony formation, sphere formation and tumor growth, and suppresses apoptosis; circ_0005962 knockdown inhibits LUAD cell growth and stemness in vitro and in vivo. |
| Molecular mechanism: |
circ_0005962 acts as a ceRNA by sponging miR-3611, thereby modulating CYP24A1 expression in LUAD cells. |
| Biological pathway or process: |
ceRNA regulation (other); proliferation (promotes); cell cycle (promotes); apoptosis (inhibits); stemness (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; RNase R Treatment; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; Transfection; CCK8; EdU Staining; Colony Formation Assay; Cell Cycle Assay; Western Blot; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); RNA Pull-Down; In Vivo Animal Model; IHC (Immunohistochemistry); Bioinformatics Analysis |
| Clinical significance: |
circ_0005962 expression was associated with TNM grade, lymph node metastasis, and tumor size in LUAD patients. |
| Description: |
circ_0005962 is up-regulated in LUAD tissues and cell lines and is associated with advanced clinicopathologic features. Functionally, it promotes LUAD cell proliferation, cell cycle progression, stem-like sphere formation and xenograft tumor growth. Mechanistically, circ_0005962 functions as a ceRNA that sponges miR-3611 to increase CYP24A1 expression. |
| Confidence score: |
0.8324 |