circRNA basic information
circBase ID: hsa_circ_0125597
Name: hsa_circ_FBXL5
Synonym: circFBXL5
Host Gene: FBXL5
Genomic location(hg19): chr4:15632288-15646331:.
Genomic location(hg38): chr4:15630665-15644708:.
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0007254
MONDO name: breast cancer
Disease details: breast cancer
Disease DO ID:
 1612
Disease MeSH ID:
 -
Disease NCIt ID:
 C9335
Disease ICD11 ID:
 1047754165
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

primary breast cancer tissues; lung metastatic tissues; breast cancer tissues
Cell lines:

MDA‐MB‐453; MDA‐MB‐231
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: SRSF6, Ago2
Associated microRNA: miR-660
Biological function: promotes breast cancer cell proliferation and lung metastasis/migration; knockdown suppresses tumour growth and lung metastasis
Molecular mechanism: acts as a miR-660 sponge and functions as a ceRNA to increase SRSF6 expression
Biological pathway or process:

proliferation (promotes); migration (promotes); metastasis (promotes); ceRNA regulation (promotes)
Detected method:
Q
M
Validation methods:

Microarray; RT-qPCR; Nuclear-Cytoplasmic Fractionation; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; Transfection; CCK8; Colony Formation Assay; In Vivo Animal Model; H&E Staining; Survival Analysis; Bioinformatics Analysis
Clinical significance:

high levels of circFBXL5 correlated with worse outcome of breast cancer
Description:

circFBXL5 is up-regulated in breast cancer (notably in lung metastatic tissues) and its high expression is associated with worse patient outcome. Functionally, circFBXL5 promotes proliferation and lung metastasis/migration, and mechanistically acts as a ceRNA sponge for miR-660 to increase SRSF6 expression via the circFBXL5/miR-660/SRSF6 axis.
Confidence score:

0.7404
Other information
Title:

circFBXL5 promotes breast cancer progression by sponging miR-660.

Journal: Journal of cellular and molecular medicine
Published: 2020
PubMed ID: 31729134
Study type:

combined biological and clinical study
Data availability: The datasets used and analysed during the current study are available from the corresponding author on reasonable request.
Code availability: -