circRNA basic information
circBase ID: -
Name: hsa_circ_CDR1as
Synonym: circular RNA ciRS-7 / ciRS-7
Host Gene: -
Genomic location(hg19): -
Genomic location(hg38): -
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0005595
MONDO name: laryngeal squamous cell carcinoma
Disease details: laryngeal squamous cell carcinoma / LSCC
Disease DO ID:
 2876
Disease MeSH ID:
 -
Disease NCIt ID:
 C4044
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

LSCC tissues; adjacent normal tissues
Cell lines:

HN-4; FD-LSC-1; UM-SCC-10A; NRECs; HEK-293 T
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: DNMT3B, TGM3, AGO2
Associated microRNA: miR-432-5p
Biological function: Promotes LSCC cell viability, growth, migration, invasion and EMT; inhibits apoptosis; promotes xenograft tumor growth.
Molecular mechanism: ciRS-7 acts as a ceRNA/miRNA sponge for miR-432-5p, relieving repression of DNMT3B, which induces TGM3 promoter hypermethylation and suppresses TGM3 expression.
Biological pathway or process:

ceRNA regulation (promotes); proliferation (promotes); migration (promotes); invasion (promotes); EMT (promotes); apoptosis (inhibits); other pathway/process (promotes)
Detected method:
Q
Validation methods:

Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; RT-qPCR; FISH / smFISH; Clinical Sample Validation; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; Transfection; CCK8; Annexin V/PI Flow Cytometry; Transwell Assay; Western Blot; MSP (Methylation-Specific PCR); In Vivo Animal Model; Bioinformatics Analysis
Clinical significance:

Higher ciRS-7 expression was associated with higher clinical stage, infiltration, and lymph node metastasis in LSCC patients.
Description:

ciRS-7 is up-regulated in LSCC tissues and cell lines and is associated with advanced clinical stage, infiltration, and lymph node metastasis. Functionally, ciRS-7 promotes LSCC cell viability, migration, invasion, EMT, and xenograft tumor growth while inhibiting apoptosis. Mechanistically, ciRS-7 sponges miR-432-5p to increase DNMT3B expression, leading to TGM3 hypermethylation and suppression.
Confidence score:

0.8275
Other information
Title:

Circular RNA ciRS-7 promotes laryngeal squamous cell carcinoma development by inducing TGM3 hypermethylation via miR-432-5p/DNMT3B axis.

Journal: Pathology, research and practice
Published: 2022
PubMed ID: 36356335
Study type:

combined biological and clinical study
Data availability: Supplementary data associated with this article can be found in the online version at doi:10.1016/j.prp.2022.154193.
Code availability: -