circRNA basic information
circBase ID: hsa_circ_0002099
Name: hsa_circ_SEMA5A
Synonym: Circ_0002099
Host Gene: SEMA5A
Genomic location(hg19): chr5:9190378-9238002:-
Genomic location(hg38): chr5:9190266-9237890:-
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0004986
MONDO name: urinary bladder carcinoma
Disease details: bladder cancer / BCa
Disease DO ID:
 4007
Disease MeSH ID:
 -
Disease NCIt ID:
 C4912
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

BLCA tissues; matched adjacent normal tissues; tumor tissues
Cell lines:

J82; T24; UMUC3; 5637; SV-HUC-1; 293 T
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: KRAS, FUS, pre-SEMA5A mRNA, WNT/beta-catenin axis
Associated microRNA: miR-217-5p, miR-103a-3p
Biological function: promotes BLCA progression, proliferation, migration, invasion, tumorigenesis, metastasis-related EMT process, and poorer prognosis
Molecular mechanism: FUS promotes Circ_0002099 biogenesis; cytoplasmic Circ_0002099 acts as a miR-217-5p/miR-103a-3p sponge to increase KRAS and regulate the WNT/beta-catenin pathway, facilitating EMT in BLCA
Biological pathway or process:

Wnt/beta-catenin (promotes); EMT (promotes); proliferation (promotes); migration (promotes); invasion (promotes); metastasis (promotes); ceRNA regulation (promotes); mRNA stability (other)
Detected method:
Q
Validation methods:

Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; RT-qPCR; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; Transfection; CCK8; Colony Formation Assay; Transwell Assay; Wound Healing Assay; In Vivo Animal Model; IHC (Immunohistochemistry); Western Blot; Survival Analysis; Bioinformatics Analysis
Clinical significance:

high Circ_0002099 expression is associated with tumor grade and poorer prognosis; it could be a promising prognostic biomarker and therapeutic target for BLCA
Description:

Circ_0002099 is a SEMA5A-derived circRNA that is upregulated in bladder cancer tissues and cell lines, and high expression is associated with poorer prognosis and tumor grade. Functionally, Circ_0002099 promotes BLCA proliferation, migration, invasion, tumorigenesis, and EMT. Mechanistically, FUS promotes Circ_0002099 biogenesis, while cytoplasmic Circ_0002099 sponges miR-217-5p and miR-103a-3p to increase KRAS and activate the WNT/beta-catenin-related EMT program.
Confidence score:

0.8768
Other information
Title:

Circ_0002099 is a novel molecular therapeutic target for bladder cancer.

Journal: Drug development research
Published: 2022
PubMed ID: 36301978
Study type:

combined biological and clinical study
Data availability: The data that support the findings of this study are available from the corresponding author upon reasonable request; Supporting Information: Table 1
Code availability: -