| Expression pattern: |
UP |
| Associated gene: |
IL6ST, JAK1, STAT3, AGO2 |
| Associated microRNA: |
miR-622 |
| Biological function: |
suppresses cell proliferation and DNA synthesis; facilitates cell apoptosis in LPS-induced A549 and Beas-2B cell injury |
| Molecular mechanism: |
circUBXN7 functions as a miR-622 sponge, indirectly upregulates IL6ST, and activates the JAK1/STAT3 signaling pathway |
| Biological pathway or process: |
JAK/STAT (promotes); apoptosis (promotes); proliferation (inhibits); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
Sanger Sequencing; RNase R Treatment; RT-qPCR; FISH / smFISH; Bioinformatics Analysis; RNA Pull-Down; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); Transfection; CCK8; EdU Staining; Annexin V/PI Flow Cytometry; Western Blot; IF (Immunofluorescence) |
| Clinical significance: |
CircUBXN7 might be a potential biomarker for ARDS and a potential therapeutic target, but clinical samples and prognosis were not analyzed. |
| Description: |
circUBXN7 is up-regulated in LPS-induced A549 and Beas-2B cell injury, an in vitro model relevant to ARDS. It suppresses cell proliferation and DNA synthesis while promoting apoptosis by sponging miR-622, increasing IL6ST expression, and activating the JAK1/STAT3 signaling pathway. The study proposes circUBXN7 as a potential ARDS biomarker and therapeutic target, although no patient cohort or in vivo validation was performed. |
| Confidence score: |
0.7822 |