| Expression pattern: |
UP |
| Associated gene: |
IL-6, Ago2 |
| Associated microRNA: |
miR-574-3p |
| Biological function: |
Promotes doxorubicin resistance in osteosarcoma; knockdown reduces DOX-resistant cell IC50 and suppresses resistance. |
| Molecular mechanism: |
hsa_circ_0010220 acts as a miR-574-3p sponge to upregulate IL-6, thereby increasing DOX resistance in osteosarcoma. |
| Biological pathway or process: |
chemoresistance (promotes); drug resistance (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Clinical Sample Validation; Bioinformatics Analysis; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); RNA Pull-Down; Transfection; CCK8; ELISA |
| Clinical significance: |
Potential biomarker and therapeutic marker for improving chemotherapy in osteosarcoma patients. |
| Description: |
hsa_circ_0010220 is up-regulated in DOX-resistant osteosarcoma tissues and cells. It promotes doxorubicin resistance by sponging miR-574-3p and thereby increasing IL-6 expression, making it a potential biomarker or therapeutic target for improving osteosarcoma chemotherapy. |
| Confidence score: |
0.7158 |