| Expression pattern: |
UP |
| Associated gene: |
TRIM44, JAK/STAT1 pathway, VEGFA, Ago2 |
| Associated microRNA: |
miR‐518a‐3p |
| Biological function: |
Promotes angiogenesis, proliferation and migration of HUVECs in the ovarian cancer microenvironment. |
| Molecular mechanism: |
Exosomal circNFIX acts as a miRNA sponge for miR-518a-3p, thereby upregulating TRIM44 and activating the JAK/STAT1 pathway to promote angiogenesis. |
| Biological pathway or process: |
angiogenesis (promotes); proliferation (promotes); migration (promotes); JAK/STAT (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Clinical Sample Validation; Bioinformatics Analysis; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); RNA Pull-Down; Transfection; MTT; Transwell Assay; Tube Formation Assay; Western Blot |
| Clinical significance: |
Upregulated circNFIX was significantly correlated with tumor size and FIGO stage of OC patients. |
| Description: |
In ovarian cancer, circNFIX is up-regulated and enriched in OC-cell-derived exosomes. Exosomal circNFIX promotes HUVEC proliferation, migration and angiogenesis by sponging miR-518a-3p, thereby increasing TRIM44 and activating the JAK/STAT1 signaling pathway. |
| Confidence score: |
0.7277 |