| Expression pattern: |
UP |
| Associated gene: |
SGMS2 |
| Associated microRNA: |
miR-485-3p |
| Biological function: |
promotes proliferation and migration of keloid fibroblasts |
| Molecular mechanism: |
ceRNA mechanism; circ_0000129 sponges miR-485-3p and releases SGMS2 expression from miR-485-3p suppression |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RT-qPCR; Clinical Sample Validation; Nuclear-Cytoplasmic Fractionation; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); Transfection; CCK8; EdU Staining; Wound Healing Assay; Transwell Assay; Western Blot; Bioinformatics Analysis |
| Clinical significance: |
targeting circ_0000129/miR-485-3p/SGMS2 might be a promising strategy against keloid fibroblasts |
| Description: |
circ_0000129 is up-regulated in keloid tissues and keloid fibroblasts. It promotes keloid fibroblast proliferation and migration through a ceRNA mechanism in which it sponges miR-485-3p and relieves miR-485-3p-mediated suppression of SGMS2. Knockdown of circ_0000129 attenuates these cellular phenotypes, suggesting potential therapeutic relevance for keloid. |
| Confidence score: |
0.7795 |