circRNA basic information
circBase ID: hsa_circ_0003028
Name: hsa_circ_FUT8
Synonym: circFUT8
Host Gene: FUT8
Genomic location(hg19): chr14:66028054-66028484:+
Genomic location(hg38): chr14:65561336-65561766:+
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0004986
MONDO name: urinary bladder carcinoma
Disease details: bladder cancer
Disease DO ID:
 4007
Disease MeSH ID:
 -
Disease NCIt ID:
 C4912
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

BCa tissues; adjacent normal bladder tissues
Cell lines:

SV-HUC-1; T24; UM-UC-3
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
DN
Associated gene: KLF10, Slug, DHX9, AGO2
Associated microRNA: miR-570-3p
Biological function: inhibits migration and invasion; inhibits lymph node metastasis
Molecular mechanism: circFUT8 acts as a miRNA sponge for miR-570-3p to promote KLF10 expression, forming the circFUT8/miR-570-3p/KLF10/Slug axis.
Biological pathway or process:

migration (inhibits); invasion (inhibits); metastasis (inhibits); EMT (inhibits); ceRNA regulation (promotes)
Detected method:
Q
S
Validation methods:

RNA-seq; RT-qPCR; Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; Actinomycin D / DRB Stability Assay; Nuclear-Cytoplasmic Fractionation; FISH / smFISH; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; RNA Pull-Down; Transfection; Transwell Assay; Wound Healing Assay; Western Blot; In Vivo Animal Model; H&E Staining; IHC (Immunohistochemistry); Clinical Sample Validation; Cohort Study; Survival Analysis; Bioinformatics Analysis
Clinical significance:

Low expression of circFUT8 in BCa patients was associated with poor prognosis, high histological grade, and LN metastasis.
Description:

circFUT8 (hsa_circ_0003028), derived from FUT8, is down-regulated in bladder cancer and mainly localized in the cytoplasm. It suppresses migration, invasion and lymph node metastasis by acting as a ceRNA that sponges miR-570-3p, thereby increasing KLF10 and modulating the KLF10/Slug (EMT-related) program. Low circFUT8 expression is associated with lymph node metastasis, higher histological grade, and worse overall survival.
Confidence score:

0.8978
Other information
Title:

circRNA circFUT8 Upregulates Krüpple-like Factor 10 to Inhibit the Metastasis of Bladder Cancer via Sponging miR-570-3p.

Journal: Molecular therapy oncolytics
Published: 2020
PubMed ID: 32072011
Study type:

combined biological and clinical study
Data availability: GEO97239
Code availability: -