circRNA basic information
circBase ID: hsa_circ_0001756
Name: hsa_circ_HIPK2
Synonym: circHIPK2
Host Gene: HIPK2
Genomic location(hg19): chr7:139415730-139416814:-
Genomic location(hg38): chr7:139715931-139717015:-
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
0001134
MONDO name: essential hypertension
Disease details: essential hypertension / EH
Disease DO ID:
10825
Disease MeSH ID:
D000075222
Disease NCIt ID:
-
Disease ICD11 ID:
761947693
Disease OMIM ID:
-
Species: Human
Species details: Homo sapiens
Tissue specimen:

thoracic aorta tissues; aortic tissues

Cell lines:

T/G HA-VSMC; 293 T

In vivo animal model:

-

circRNA-disease information
Expression pattern:
UP
Associated gene: ADAM17, AGO2
Associated microRNA: miR-145-5p
Biological function: Promotes AngII-induced VSMC phenotype switching, promotes VSMC proliferation, and inhibits VSMC apoptosis.
Molecular mechanism: circHIPK2 acts as a miR-145-5p sponge to upregulate ADAM17 expression, forming a circHIPK2/miR-145-5p/ADAM17 axis.
Biological pathway or process:

proliferation (promotes); apoptosis (inhibits); ceRNA regulation (promotes); other pathway/process (promotes)

Detected method:
Q
H
Validation methods:

RNase R Treatment; Actinomycin D / DRB Stability Assay; RT-qPCR; FISH / smFISH; IF (Immunofluorescence); Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; Transfection; CCK8; Colony Formation Assay; Annexin V/PI Flow Cytometry; Western Blot; Bioinformatics Analysis

Clinical significance:

circHIPK2 may represent a new therapeutic target for hypertension.

Description:

circHIPK2 is a human HIPK2-derived circRNA that is upregulated in aortic VSMCs from hypertensive patients. It promotes AngII-induced VSMC phenotype switching by sponging miR-145-5p and increasing ADAM17 expression, thereby enhancing VSMC proliferation and suppressing apoptosis. The study suggests circHIPK2 as a potential therapeutic target for hypertension.

Confidence score:

0.8203

Other information
Title:

CircHIPK2 facilitates phenotypic switching of vascular smooth muscle cells in hypertension.

Journal: Journal of human hypertension
Published: 2023
PubMed ID: 37100987
Study type:

combined biological and clinical study

Data availability: The data that support the findings of this study are available from the corresponding author upon reasonable request; supplementary material available at https://doi.org/10.1038/s41371-023-00834-w
Code availability: -