| Expression pattern: |
UP |
| Associated gene: |
MAPK8, EIF4A3, AGO2 |
| Associated microRNA: |
miR-214-3p |
| Biological function: |
Promotes migration and invasion of NSCLC cells. |
| Molecular mechanism: |
circTADA2A sponges miR-214-3p to up-regulate MAPK8 and recruits EIF4A3 to stabilize MAPK8 mRNA. |
| Biological pathway or process: |
migration (promotes); invasion (promotes); ceRNA regulation (promotes); mRNA stability (promotes) |
| Detected method: |
Q
M
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; RT-qPCR; FISH / smFISH; RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; Transfection; Wound Healing Assay; Transwell Assay; Western Blot; Bioinformatics Analysis |
| Clinical significance: |
circTADA2A might serve as a novel potential biomarker for NSCLC. |
| Description: |
circTADA2A is highly expressed in NSCLC cells and functions as an oncogenic circRNA that promotes migration and invasion. Mechanistically, it up-regulates MAPK8 through two routes: sponging miR-214-3p in a ceRNA manner and recruiting the RBP EIF4A3 to stabilize MAPK8 mRNA. |
| Confidence score: |
0.7785 |