| Expression pattern: |
DN |
| Associated gene: |
ELAVL1/HuR, ANO7, WTAP, METTL3, METTL14, FTO, HNRNPD, SART3, FUBP2, PUF60 |
| Associated microRNA: |
- |
| Biological function: |
Promotes apoptosis and inhibits proliferation, migration/metastasis, and tumor growth in prostate cancer (tumor suppressor). |
| Molecular mechanism: |
circDDIT4 acts as an RBP sponge by competitively binding ELAVL1/HuR via its 3′-UTR, thereby reducing ELAVL1 target mRNA stability/expression (including ANO7); its biogenesis is promoted by m6A modification mediated by WTAP/METTL3/METTL14 and inhibited by FTO demethylation. |
| Biological pathway or process: |
apoptosis (promotes); proliferation (inhibits); migration (inhibits); metastasis (inhibits); m6A modification (other); mRNA stability (inhibits) |
| Detected method: |
Q
B
|
| Validation methods: |
RT-qPCR; Back-Splice Junction PCR / divergent primers PCR; Sanger Sequencing; Northern Blot; Nuclear-Cytoplasmic Fractionation; RNase R Treatment; Actinomycin D / DRB Stability Assay; RNA-seq; Clinical Sample Validation; Transfection; RNA Pull-Down; RIP (RNA Immunoprecipitation); Western Blot; MeRIP / MeRIP-seq; Co-IP; CCK8; Colony Formation Assay; Annexin V/PI Flow Cytometry; Transwell Assay; Wound Healing Assay; In Vivo Animal Model |
| Clinical significance: |
Lower circDDIT4 expression in prostate cancer tissues and serum suggests circDDIT4 as a potential biomarker or therapeutic target for prostate cancer. |
| Description: |
circDDIT4 is down-regulated in prostate cancer tissues, serum, and cell lines and acts as a tumor suppressor. Mechanistically, circDDIT4 sponges the RBP ELAVL1/HuR via its 3′-UTR, reducing ELAVL1-dependent expression/stability of targets such as ANO7, thereby promoting apoptosis and inhibiting proliferation and migration/metastasis. Its circularization/biogenesis is promoted by WTAP/METTL3/METTL14-mediated m6A modification and antagonized by FTO. |
| Confidence score: |
0.8524 |