| Expression pattern: |
UP |
| Associated gene: |
AKT3 mRNA, AKT3, mTOR, ZNF609-250aa |
| Associated microRNA: |
- |
| Biological function: |
circ-ZNF609 promotes renal tubular epithelial cell apoptosis and acute kidney injury, impairs autophagic flux, suppresses proliferation, and its silencing is renoprotective in ischemic AKI. |
| Molecular mechanism: |
circ-ZNF609 encodes a 250-amino-acid protein, ZNF609-250aa, through an IRES-dependent mechanism; ZNF609-250aa activates AKT3/mTOR signaling, impairs autophagic flux, and promotes apoptosis in ischemic AKI. |
| Biological pathway or process: |
PI3K/AKT/mTOR (promotes); autophagy (inhibits); apoptosis (promotes); proliferation (inhibits); other pathway/process (promotes) |
| Detected method: |
Q
H
S
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; RT-qPCR; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; RNA-seq; Transfection; MTS; Annexin V/PI Flow Cytometry; TUNEL; In Vivo Animal Model; H&E Staining; Western Blot; Luciferase Reporter Assay; ROC Analysis; Bioinformatics Analysis; Droplet digital PCR |
| Clinical significance: |
Urinary circ-ZNF609 is a moderate predictor for AKI in patients with acute decompensated heart failure or undergoing cardiac surgery under cardiopulmonary bypass, with an AUC of 0.795. |
| Description: |
circ-ZNF609 is up-regulated in ischemic AKI kidney tissues and in urinary samples from AKI patients. It encodes the 250-aa protein ZNF609-250aa, which activates AKT3/mTOR signaling, impairs autophagic flux, promotes renal tubular epithelial cell apoptosis, suppresses proliferation, and contributes to AKI; urinary circ-ZNF609 also shows moderate diagnostic potential for AKI. |
| Confidence score: |
0.8441 |