circRNA basic information
circBase ID: hsa_circ_0000119
Name: hsa_circ_MAN1A2
Synonym: circRNA mannosidase alpha class 1A member 2
Host Gene: MAN1A2
Genomic location(hg19): chr1:117944807-117984947:+
Genomic location(hg38): chr1:117402185-117442325:+
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0005075
MONDO name: thyroid gland papillary carcinoma
Disease details: papillary thyroid carcinoma / PTC
Disease DO ID:
 3969
Disease MeSH ID:
 -
Disease NCIt ID:
 C4035
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

PTC samples; adjacent normal samples; PTC tissues; adjacent normal tissues; xenograft tumor tissues
Cell lines:

Nthy-ori 3-1; TPC-1; FTC-133; SW579
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: MTDH, Ago2, MAN1A2
Associated microRNA: miR-449a
Biological function: CircMAN1A2 promotes proliferation, migration, invasion, EMT and tumor growth of papillary thyroid carcinoma cells; circMAN1A2 silencing inhibits these malignant behaviors.
Molecular mechanism: CircMAN1A2 functions as a miR-449a sponge and upregulates MTDH expression through the circMAN1A2/miR-449a/MTDH ceRNA axis.
Biological pathway or process:

ceRNA regulation (promotes); proliferation (promotes); migration (promotes); invasion (promotes); EMT (promotes); metastasis (promotes)
Detected method:
Q
Validation methods:

RT-qPCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; Clinical Sample Validation; Bioinformatics Analysis; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); Transfection; Colony Formation Assay; EdU Staining; Wound Healing Assay; Transwell Assay; Western Blot; In Vivo Animal Model; IHC (Immunohistochemistry)
Clinical significance:

CircMAN1A2 is aberrantly upregulated in PTC tissues and may be a potential therapeutic target through the circ-MAN1A2/miR-449a/MTDH axis.
Description:

circMAN1A2/hsa_circ_0000119 is upregulated in papillary thyroid carcinoma tissues and cell lines. It acts as an oncogenic circRNA that promotes PTC cell proliferation, migration, invasion, EMT and xenograft tumor growth by sponging miR-449a and thereby increasing MTDH expression. The study suggests the circ-MAN1A2/miR-449a/MTDH axis as a potential therapeutic target for PTC.
Confidence score:

0.8298
Other information
Title:

circRNA mannosidase alpha class 1A member 2 contributes to the proliferation and motility of papillary thyroid cancer cells through upregulating metadherin via absorbing microRNA-449a.

Journal: Anti-cancer drugs
Published: 2023
PubMed ID: 36066401
Study type:

combined biological and clinical study
Data availability: available from the corresponding author on reasonable request
Code availability: -