| Expression pattern: |
UP |
| Associated gene: |
CLDN1, C-myc, MMP2 |
| Associated microRNA: |
miR-129-5p |
| Biological function: |
Promotes PTC cell proliferation, viability, migration, invasion and tumorigenesis; inhibits apoptosis; high expression is associated with poor prognosis and diagnostic value. |
| Molecular mechanism: |
circ_0027446 acts as a miR-129-5p sponge and upregulates CLDN1 expression through the miR-129-5p/CLDN1 pathway. |
| Biological pathway or process: |
ceRNA regulation (promotes); proliferation (promotes); migration (promotes); invasion (promotes); apoptosis (inhibits); metastasis (promotes) |
| Detected method: |
Q
M
|
| Validation methods: |
RT-qPCR; RNase R Treatment; Clinical Sample Validation; Bioinformatics Analysis; ROC Analysis; Survival Analysis; Transfection; CCK8; EdU Staining; Colony Formation Assay; Annexin V/PI Flow Cytometry; Transwell Assay; Wound Healing Assay; Western Blot; Luciferase Reporter Assay; In Vivo Animal Model; IHC (Immunohistochemistry) |
| Clinical significance: |
High circ_0027446 expression was closely associated with poor prognosis of PTC patients and showed diagnostic value for distinguishing PTC from normal control. |
| Description: |
circ_0027446 is upregulated in papillary thyroid cancer tissues and cells and functions as an oncogenic circRNA. It promotes PTC proliferation, migration, invasion and xenograft tumor growth while suppressing apoptosis, mainly by sponging miR-129-5p to increase CLDN1 expression. High circ_0027446 expression is associated with poor patient survival and may have diagnostic and therapeutic relevance. |
| Confidence score: |
0.7987 |