| Expression pattern: |
UP |
| Associated gene: |
MAPK1, AGO2 protein, P-ERK1/2, ERK1/2 |
| Associated microRNA: |
miR-330-5p |
| Biological function: |
Functions as an oncogene in CRC; promotes CRC cell proliferation, migration, and invasion; knockdown suppresses cell proliferation, migration, invasion, cell viability, colony formation, PCNA expression, and CRC progression. |
| Molecular mechanism: |
circ_0071803 acts as a miR-330-5p sponge to upregulate MAPK1 and enhance MAPK signaling, thereby promoting CRC progression. |
| Biological pathway or process: |
MAPK (promotes); ERK (promotes); proliferation (promotes); migration (promotes); invasion (promotes); metastasis (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
M
|
| Validation methods: |
RT-qPCR; RNase R Treatment; Clinical Sample Validation; RIP (RNA Immunoprecipitation); Transfection; CCK8; Colony Formation Assay; Transwell Assay; Western Blot; Survival Analysis; Bioinformatics Analysis |
| Clinical significance: |
circ_0071803 upregulation predicts poor prognosis and is inversely correlated with survival rate in patients with CRC; it may be a potential therapeutic target for CRC treatment. |
| Description: |
circ_0071803 is upregulated in colorectal cancer tissues and cell lines, and higher expression is associated with poorer survival. Functionally, circ_0071803 promotes CRC cell proliferation, migration, and invasion by sponging miR-330-5p to increase MAPK1 expression and activate MAPK signaling, suggesting its potential as a therapeutic target. |
| Confidence score: |
0.7646 |