circRNA basic information
circBase ID: hsa_circ_0000291
Name: hsa_circ_CD44
Synonym: circ-CD44 / hsa-circRNA-000792
Host Gene: CD44
Genomic location(hg19): chr11:35163017-35163328:+
Genomic location(hg38): chr11:35141470-35141781:+
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0005575
MONDO name: colorectal cancer
Disease details: colorectal cancer / CRC
Disease DO ID:
 5672, 9256
Disease MeSH ID:
 -
Disease NCIt ID:
 C4978
Disease ICD11 ID:
 -
Disease OMIM ID:
 114500
Species: Human
Species details: Homo sapiens
Tissue specimen:

CRC tissues; paired adjacent tissues; OXA-resistant CRC tissues; dissected tumor tissues
Cell lines:

SW1116; Lovo; SW620; HCT-116; NCM460; SW620/OXA; HCT116/OXA; HCT-116/OXA
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: ABCC1, Ago2
Associated microRNA: miR-330-5p
Biological function: Circ-CD44 promotes OXA resistance, proliferation, migration, invasion, and tumor growth, and inhibits apoptosis in OXA-resistant colorectal cancer cells.
Molecular mechanism: Circ-CD44 acts as a miRNA sponge for miR-330-5p, thereby restoring/upregulating ABCC1 expression and regulating oxaliplatin chemotherapy resistance.
Biological pathway or process:

chemoresistance (promotes); proliferation (promotes); migration (promotes); invasion (promotes); apoptosis (inhibits); ceRNA regulation (promotes)
Detected method:
Q
Validation methods:

RT-qPCR; Clinical Sample Validation; Transfection; MTT; Colony Formation Assay; Annexin V/PI Flow Cytometry; Transwell Assay; In Vivo Animal Model; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); Western Blot; Bioinformatics Analysis
Clinical significance:

Circ-CD44 was upregulated in OXA-resistant CRC tissues and the circ-CD44/miR-330-5p/ABCC1 axis might have potential use in OXA-resistant CRC therapy.
Description:

Circ-CD44 is upregulated in colorectal cancer, especially in oxaliplatin-resistant CRC tissues and cells. It promotes oxaliplatin resistance and malignant phenotypes by sponging miR-330-5p and restoring ABCC1 expression, while circ-CD44 silencing increases OXA sensitivity, induces apoptosis, and suppresses proliferation, migration, invasion, and xenograft tumor growth.
Confidence score:

0.705
Other information
Title:

Circular RNA circ-CD44 regulates chemotherapy resistance by targeting the miR-330-5p/ABCC1 axis in colorectal cancer cells.

Journal: Histology and histopathology
Published: 2023
PubMed ID: 36106650
Study type:

combined biological and clinical study
Data availability: GSE126094; Please contact the corresponding author for data request.
Code availability: -