| Expression pattern: |
UP |
| Associated gene: |
TWSG1 |
| Associated microRNA: |
miR-136 |
| Biological function: |
Promotes gastric cancer cell proliferation, migration, invasion, cell cycle progression and xenograft tumorigenesis, and inhibits apoptosis. |
| Molecular mechanism: |
hsa_circ_0001013 acts as a miR-136 sponge to upregulate TWSG1 expression, forming the hsa_circ_0001013/miR-136/TWSG1 axis. |
| Biological pathway or process: |
ceRNA regulation (promotes); proliferation (promotes); migration (promotes); invasion (promotes); apoptosis (inhibits); cell cycle (promotes); angiogenesis (promotes) |
| Detected method: |
Q
M
|
| Validation methods: |
RT-qPCR; Microarray; Northern Blot; FISH / smFISH; Clinical Sample Validation; RNA Pull-Down; Luciferase Reporter Assay; Transfection; CCK8; EdU Staining; Colony Formation Assay; Annexin V/PI Flow Cytometry; Cell Cycle Assay; Transwell Assay; Wound Healing Assay; In Vivo Animal Model; IHC (Immunohistochemistry); Western Blot; Cohort Study; Survival Analysis; Bioinformatics Analysis |
| Clinical significance: |
High circ_0001013 expression is associated with poor prognosis in gastric cancer patients. |
| Description: |
hsa_circ_0001013 is upregulated in gastric cancer tissues and cell lines and its high expression is associated with poor patient survival. Functionally, it promotes proliferation, migration, invasion, cell cycle progression and xenograft tumor growth while inhibiting apoptosis. Mechanistically, hsa_circ_0001013 binds miR-136 and increases TWSG1 expression through a circRNA-miRNA-mRNA regulatory axis. |
| Confidence score: |
0.8192 |