| Expression pattern: |
UP |
| Associated gene: |
KRT5 |
| Associated microRNA: |
miR-520a-5p |
| Biological function: |
Promotes NSCLC cell proliferation, migration, invasion, and tumor growth; silencing inhibits these phenotypes. |
| Molecular mechanism: |
Acts as a competing endogenous RNA binding miR-520a-5p to regulate KRT5 expression. |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); metastasis (promotes); EMT (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; RNase R Treatment; Clinical Sample Validation; Transfection; EdU Staining; Colony Formation Assay; MTT; Transwell Assay; Western Blot; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); In Vivo Animal Model; IHC (Immunohistochemistry) |
| Clinical significance: |
providing a potential new target for the treatment and diagnosis of NSCLC |
| Description: |
hsa_circ_0017620 is up-regulated in NSCLC tissues and cell lines and promotes proliferation, migration/invasion and in vivo tumor growth. Mechanistically, it functions as a ceRNA by sponging miR-520a-5p to increase KRT5, and miR-520a-5p inhibition or KRT5 overexpression rescues the effects of hsa_circ_0017620 knockdown. |
| Confidence score: |
0.7856 |