circRNA basic information
circBase ID: hsa_circ_0065898
Name: hsa_circ_VPRBP
Synonym: circRNA DDB1-and CUL4-associated factor 1 / circVPRBP
Host Gene: VPRBP
Genomic location(hg19): chr3:51454240-51456330:-
Genomic location(hg38): chr3:51416786-51418876:-
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0002974
MONDO name: cervical cancer
Disease details: cervical cancer / CC
Disease DO ID:
 4362
Disease MeSH ID:
 -
Disease NCIt ID:
 C9311
Disease ICD11 ID:
 1256072522
Disease OMIM ID:
 603956
Species: Human
Species details: Homo sapiens
Tissue specimen:

fresh cervical cancer tissue samples; matched adjacent normal tissue samples; mice tumor tissues
Cell lines:

Ect1/E6E7; CaSki; SiHa
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
DN
Associated gene: TRIM3, MMP2, MMP9, Ago2
Associated microRNA: miR-106b-5p
Biological function: CircVPRBP suppresses cervical cancer cell growth, proliferation, migration, invasion and metastasis, promotes apoptosis, arrests cell cycle progression, and represses tumor growth in vivo.
Molecular mechanism: CircVPRBP acts as a cytoplasmic miR-106b-5p sponge and regulates TRIM3 expression through a ceRNA mechanism.
Biological pathway or process:

ceRNA regulation (other); proliferation (inhibits); apoptosis (promotes); cell cycle (inhibits); migration (inhibits); invasion (inhibits); metastasis (inhibits)
Detected method:
Q
M
Validation methods:

Microarray; RT-qPCR; Clinical Sample Validation; Actinomycin D / DRB Stability Assay; RNase R Treatment; Nuclear-Cytoplasmic Fractionation; Bioinformatics Analysis; Transfection; MTT; EdU Staining; Colony Formation Assay; Annexin V/PI Flow Cytometry; Cell Cycle Assay; Transwell Assay; Western Blot; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); In Vivo Animal Model
Clinical significance:

CircVPRBP may provide a promising therapeutic target and preclinical basis for cervical cancer treatment.
Description:

CircVPRBP/hsa_circ_0065898 is down-regulated in cervical cancer tissues and cell lines. It functions as a tumor suppressive circRNA by sponging miR-106b-5p to increase TRIM3 expression, thereby inhibiting proliferation, cell cycle progression, migration, invasion, metastasis, and in vivo tumor growth while promoting apoptosis. The study suggests circVPRBP may be a potential therapeutic target for cervical cancer.
Confidence score:

0.8207
Other information
Title:

Circular RNA circVPRBP serves as a microRNA-106b-5p sponge to regulate proliferation and metastasis of cervical cancer cells via tripartite motif-containing protein 3.

Journal: Anti-cancer drugs
Published: 2022
PubMed ID: 35946546
Study type:

combined biological and clinical study
Data availability: GSE102686; TCGA CESC; please contact the corresponding author for the data request
Code availability: -