circRNA basic information
circBase ID: hsa_circ_0021977
Name: hsa_circ_PSMC3
Synonym: circ_0021977
Host Gene: PSMC3
Genomic location(hg19): chr11:47440319-47442278:-
Genomic location(hg38): chr11:47418768-47420727:-
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0005575
MONDO name: colorectal cancer
Disease details: colorectal cancer / CRC
Disease DO ID:
 5672, 9256
Disease MeSH ID:
 -
Disease NCIt ID:
 C4978
Disease ICD11 ID:
 -
Disease OMIM ID:
 114500
Species: Human
Species details: Homo sapiens
Tissue specimen:

plasma; preoperative blood samples; CRC tissues; adjacent normal tissues
Cell lines:

SW480; HCT8; HCT116; DLD1; HCoEpic; 293 T
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
DN
Associated gene: p21, p53, P21, P53, CDKN1A, TP53
Associated microRNA: miR-10b-5p
Biological function: circ_0021977 acts as a tumor suppressor in CRC and inhibits CRC cell proliferation, migration, invasion, and metastasis in vitro, and suppresses CRC cell proliferation in vivo.
Molecular mechanism: circ_0021977 functions as a ceRNA/miRNA sponge for miR-10b-5p, thereby regulating P21/P53 expression and suppressing CRC proliferation, migration, and invasion.
Biological pathway or process:

proliferation (inhibits); migration (inhibits); invasion (inhibits); metastasis (inhibits); ceRNA regulation (other); p53 signaling (promotes)
Detected method:
Q
Validation methods:

Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; RT-qPCR; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; RNA Pull-Down; Luciferase Reporter Assay; Transfection; CCK8; EdU Staining; Colony Formation Assay; Transwell Assay; In Vivo Animal Model; IHC (Immunohistochemistry); Western Blot; Cohort Study; Survival Analysis; ROC Analysis; Bioinformatics Analysis
Clinical significance:

Low circ_0021977 expression is associated with higher TNM stage, lymph node metastasis, larger tumor size, poorer prognosis, and lower overall survival; plasma circ_0021977 may serve as a noninvasive diagnostic biomarker for CRC.
Description:

circ_0021977 is a human PSMC3-derived circRNA that is downregulated in CRC tissues, plasma, and cell lines. It suppresses CRC proliferation, migration, invasion, and xenograft tumor growth by sponging miR-10b-5p and indirectly increasing P21/P53 expression. Clinically, low circ_0021977 expression is associated with lymph node metastasis, advanced TNM stage, poorer prognosis, and diagnostic potential in plasma.
Confidence score:

0.8844
Other information
Title:

The circ_0021977/miR-10b-5p/P21 and P53 regulatory axis suppresses proliferation, migration, and invasion in colorectal cancer.

Journal: Journal of cellular physiology
Published: 2020
PubMed ID: 31595500
Study type:

combined biological and clinical study
Data availability: The data used to support the findings of this study are not shared; available from the corresponding author on reasonable request
Code availability: -