circRNA basic information
circBase ID: hsa_circ_0007331
Name: hsa_circ_ACAP2
Synonym: circ_0,007,331
Host Gene: ACAP2
Genomic location(hg19): chr3:195101737-195112876:-
Genomic location(hg38): chr3:195381008-195392147:-
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0007254
MONDO name: breast cancer
Disease details: breast cancer / BC
Disease DO ID:
 1612
Disease MeSH ID:
 -
Disease NCIt ID:
 C9335
Disease ICD11 ID:
 1047754165
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

BC tumor tissues; adjacent normal tissues; adjacent nontumor tissues; tumor tissues
Cell lines:

MCF-7; SKBR3; MDA-MB-453; T47D; MCF-7/PR; SKBR3/PR; SKBR3/PR
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: ANLN, CyclinD1, Cleaved-caspase3, MMP9, Ki67
Associated microRNA: miR-200b-3p
Biological function: Contributes to PTX resistance, promotes proliferation, cell cycle progression, migration and invasion, suppresses apoptosis, and promotes xenograft tumor growth in breast cancer.
Molecular mechanism: circ_0,007,331 functions as a miRNA sponge for miR-200b-3p and positively regulates ANLN expression, thereby mediating PTX resistance and malignant phenotypes of breast cancer cells.
Biological pathway or process:

chemoresistance (promotes); drug resistance (promotes); proliferation (promotes); migration (promotes); invasion (promotes); apoptosis (inhibits); cell cycle (promotes); ceRNA regulation (promotes)
Detected method:
Q
Validation methods:

RNase R Treatment; Actinomycin D / DRB Stability Assay; RT-qPCR; Clinical Sample Validation; Transfection; CCK8; Cell Cycle Assay; Annexin V/PI Flow Cytometry; Transwell Assay; Western Blot; Bioinformatics Analysis; Luciferase Reporter Assay; RNA Pull-Down; In Vivo Animal Model; IHC (Immunohistochemistry)
Clinical significance:

circ_0,007,331 might be a potential PTX-resistant biomarker in BC cells; combined therapy of circ_0,007,331 knockdown and PTX treatment may be effective for nontriple negative BC patients.
Description:

circ_0,007,331 is up-regulated in breast cancer tissues and PTX-resistant breast cancer cell lines. It promotes PTX resistance, proliferation, cell cycle progression, migration, invasion and xenograft tumor growth while inhibiting apoptosis, at least partly by sponging miR-200b-3p to increase ANLN expression.
Confidence score:

0.8298
Other information
Title:

Circ_0,007,331 Promotes the PTX Resistance and Progression of Breast Cancer via miR-200b-3p/ANLN.

Journal: The Journal of surgical research
Published: 2022
PubMed ID: 35926312
Study type:

combined biological and clinical study
Data availability: Supplementary data to this article can be found online at https://doi.org/10.1016/j.jss.2022.05.004.
Code availability: -