circRNA basic information
circBase ID: hsa_circ_0004672
Name: hsa_circ_IPO7
Synonym: circIPO7
Host Gene: IPO7
Genomic location(hg19): chr11:9441957-9446809:+
Genomic location(hg38): chr11:9420410-9425262:+
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0015459
MONDO name: nasopharyngeal carcinoma
Disease details: nasopharyngeal carcinoma / NPC
Disease DO ID:
 9261
Disease MeSH ID:
 D00007727; D000077274
Disease NCIt ID:
 C3871
Disease ICD11 ID:
 1883313543
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

NPC tissues; normal nasopharyngeal tissues; NPC tissue specimens; lung tissues; footpad tumors; inguinal lymph nodes; subcutaneous xenograft tumors
Cell lines:

S18; S26; HONE1; HK1; CNE2; HNE1; SUNE1; C666-1; NP69; HEK293T
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: YBX1, AKT, FGFR1, TNC, NTRK1
Associated microRNA: -
Biological function: Promotes NPC metastasis, migration, invasion and cisplatin chemoresistance; suppresses cisplatin-induced DNA damage and apoptosis; knockdown suppresses metastasis and increases sensitivity to cisplatin treatment in vitro and in vivo.
Molecular mechanism: circIPO7 binds YBX1 in the cytoplasm, facilitates AKT-mediated YBX1 phosphorylation at serine 102, promotes YBX1 nuclear translocation, and activates FGFR1, TNC and NTRK1 transcription in the PI3K-AKT signaling context.
Biological pathway or process:

PI3K/AKT (promotes); migration (promotes); invasion (promotes); metastasis (promotes); drug resistance (promotes); chemoresistance (promotes); apoptosis (inhibits); other pathway/process (promotes)
Detected method:
Q
S
Validation methods:

Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; circRNA-seq; Actinomycin D / DRB Stability Assay; RT-qPCR; RNA-seq; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; RIP (RNA Immunoprecipitation); RNA Pull-Down; Co-IP; ChIP / ChIP-seq; Luciferase Reporter Assay; Transfection; CCK8; Colony Formation Assay; Transwell Assay; IF (Immunofluorescence); In Vivo Animal Model; H&E Staining; IHC (Immunohistochemistry); Western Blot; Cohort Study; Survival Analysis; ROC Analysis; Bioinformatics Analysis
Clinical significance:

High circIPO7 expression is associated with unfavorable prognosis, increased risk of distant metastasis, shorter OS, DFS and DMFS, and worse survival after cisplatin-based chemotherapy; it is an independent prognostic biomarker and potential therapeutic target.
Description:

circIPO7 (hsa_circ_0004672) is an IPO7-derived circRNA upregulated in NPC, especially in patients with distant metastasis. It promotes NPC migration, invasion, metastasis and cisplatin chemoresistance by binding YBX1 in the cytoplasm, facilitating AKT-mediated YBX1 phosphorylation and nuclear translocation, and activating FGFR1, TNC and NTRK1 transcription. Clinically, high circIPO7 expression predicts increased distant metastasis risk and poor survival after cisplatin-based chemotherapy.
Confidence score:

0.9064
Other information
Title:

CircIPO7 Promotes Nasopharyngeal Carcinoma Metastasis and Cisplatin Chemoresistance by Facilitating YBX1 Nuclear Localization.

Journal: Clinical cancer research : an official journal of the American Association for Cancer Research
Published: 2022
PubMed ID: 35917517
Study type:

combined biological and clinical study
Data availability: GSE137543; GSE189459; RDDB2022142077
Code availability: -