| Expression pattern: |
DN |
| Associated gene: |
TRIM16 |
| Associated microRNA: |
miR-942 |
| Biological function: |
inhibits cisplatin resistance, suppresses cell growth, metastasis, and glycolysis, and promotes apoptosis in CDDP-resistant NSCLC cells |
| Molecular mechanism: |
circPTK2 binds miR-942 (miRNA sponge) to relieve miR-942-mediated repression of TRIM16, thereby reducing CDDP resistance and malignant phenotypes |
| Biological pathway or process: |
proliferation (inhibits); apoptosis (promotes); migration (inhibits); invasion (inhibits); metastasis (inhibits); glycolysis (inhibits); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; Nuclear-Cytoplasmic Fractionation; FISH / smFISH; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); Transfection; MTT; Transwell Assay; Annexin V/PI Flow Cytometry; Western Blot; In Vivo Animal Model; Bioinformatics Analysis; Flow Cytometry（Non-apoptosis/cycle） |
| Clinical significance: |
- |
| Description: |
circPTK2 (hsa_circ_0008305) is down-regulated in NSCLC tissues/cell lines and further decreased in CDDP-resistant NSCLC cells. circPTK2 mainly localizes to the cytoplasm and functions as a miR-942 sponge to derepress TRIM16, thereby suppressing proliferation/migration/invasion and glycolysis, promoting apoptosis, and reducing cisplatin resistance in vitro and in a xenograft model. |
| Confidence score: |
0.7735 |