| Expression pattern: |
UP |
| Associated gene: |
SERPINB5 mRNA, SERPINB5 protein |
| Associated microRNA: |
- |
| Biological function: |
Promotes AGS gastric cancer cell proliferation and invasion in vitro; candidate oncogene in gastric cancer. |
| Molecular mechanism: |
circRNA0047905 positively correlates with and regulates its parental gene SERPINB5 expression; the authors speculate that it may modulate parental gene expression transcriptionally. |
| Biological pathway or process: |
proliferation (promotes); invasion (promotes); other pathway/process (other) |
| Detected method: |
Q
M
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RT-qPCR; Microarray; FISH / smFISH; Clinical Sample Validation; Transfection; CCK8; Transwell Assay; Western Blot; ROC Analysis; Bioinformatics Analysis |
| Clinical significance: |
circRNA0047905 had the highest diagnostic accuracy for GC with an AUC of 0.85 and may be a promising biomarker in GC diagnosis. |
| Description: |
hsa_circ_0047905/circRNA0047905 is significantly up-regulated in gastric cancer tissues and preferentially localized in the cytoplasm of AGS cells. Its knockdown suppresses AGS cell proliferation and invasion, and it positively correlates with and regulates the parental gene SERPINB5. It also shows diagnostic potential for GC, with the highest reported AUC among the three circRNAs studied. |
| Confidence score: |
0.7444 |