circRNA basic information
circBase ID: hsa_circ_0000798
Name: hsa_circ_BPTF
Synonym: -
Host Gene: BPTF
Genomic location(hg19): chr17:65941524-65944422:+
Genomic location(hg38): chr17:67945408-67948306:+
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0005086
MONDO name: renal cell carcinoma
Disease details: renal cell carcinoma / RCC
Disease DO ID:
 4450
Disease MeSH ID:
 D002292
Disease NCIt ID:
 -
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

tumor tissue; paired adjacent non-tumor tissue; tumor tissues
Cell lines:

A498; 769P; HK2
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: G3BP1, Ki67, Bcl-2, Bax, cleaved caspase-3
Associated microRNA: miR-589-5p
Biological function: Circ_0000798 exerts an oncogenic role in RCC by promoting cell metabolic viability, DNA synthesis, colony formation, wound healing, migration, invasion, and tumor growth, while inhibiting cell cycle arrest and apoptosis.
Molecular mechanism: Circ_0000798 functions as a ceRNA/miRNA sponge for miR-589-5p to regulate G3BP1 expression, forming a circ_0000798/miR-589-5p/G3BP1 regulatory axis.
Biological pathway or process:

ceRNA regulation (promotes); proliferation (promotes); migration (promotes); invasion (promotes); apoptosis (inhibits); cell cycle (promotes)
Detected method:
Q
Validation methods:

Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; RT-qPCR; Clinical Sample Validation; RNA Pull-Down; Luciferase Reporter Assay; Transfection; MTT; EdU Staining; Colony Formation Assay; Annexin V/PI Flow Cytometry; Cell Cycle Assay; Transwell Assay; Wound Healing Assay; Western Blot; IHC (Immunohistochemistry); In Vivo Animal Model; Cohort Study; Survival Analysis; Bioinformatics Analysis
Clinical significance:

High circ_0000798 is associated with shorter overall survival and might correlate with poor prognosis of RCC patients; circ_0000798 may have potential in RCC diagnosis and development.
Description:

Circ_0000798 is a BPTF-derived human circRNA upregulated in RCC tissues and cell lines, and high expression is associated with poorer overall survival. Functionally, circ_0000798 promotes RCC cell growth, colony formation, migration, invasion, and xenograft tumor growth while suppressing apoptosis. Mechanistically, it acts as a miR-589-5p sponge to increase G3BP1 expression, forming a circ_0000798/miR-589-5p/G3BP1 ceRNA axis.
Confidence score:

0.8656
Other information
Title:

Knockdown of Circ_0000798 Impedes Cell Growth and Motility of Renal Cell Carcinoma Cells Through Functioning as miRNA Sponge for miR-589-5p.

Journal: Biochemical genetics
Published: 2023
PubMed ID: 35857217
Study type:

combined biological and clinical study
Data availability: GSE137836; GSE100186; available from the corresponding author on reasonable request
Code availability: -