circRNA basic information
circBase ID: -
Name: -
Synonym: circRNA_001678 / circ_001678
Host Gene: -
Genomic location(hg19): -
Genomic location(hg38): -
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0005233
MONDO name: non-small cell lung carcinoma
Disease details: non-small cell lung cancer / NSCLC
Disease DO ID:
 3908
Disease MeSH ID:
 D002289
Disease NCIt ID:
 C2926
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

NSCLC tissues; adjacent normal tissue samples; tumor tissues
Cell lines:

NCI-H358; NCI-H1299; A549; HBE1
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: ZEB1, PD-L1, AGO2, CD8+ T cells
Associated microRNA: miR-326
Biological function: Promotes NSCLC cell proliferation, migration, invasion, in vivo tumor growth, CD8+ T cell apoptosis, immune escape, and malignant progression.
Molecular mechanism: circ_001678 acts as a miR-326 sponge to up-regulate ZEB1, activates the PD-1/PD-L1 pathway, promotes CD8+ T cell apoptosis, and induces NSCLC immune escape.
Biological pathway or process:

proliferation (promotes); migration (promotes); invasion (promotes); apoptosis (promotes); immune regulation (promotes); ceRNA regulation (other); other pathway/process (promotes)
Detected method:
Q
S
Validation methods:

circRNA-seq; RT-qPCR; Clinical Sample Validation; Transfection; CCK8; Transwell Assay; RNA Pull-Down; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; FISH / smFISH; Annexin V/PI Flow Cytometry; Flow Cytometry（Non-apoptosis/cycle）; IHC (Immunohistochemistry); In Vivo Animal Model; Bioinformatics Analysis
Clinical significance:

-
Description:

circ_001678 is up-regulated in NSCLC tissues and cells and promotes malignant phenotypes including proliferation, migration, invasion, and xenograft tumor growth. Mechanistically, it sponges miR-326 to increase ZEB1 expression, activates the PD-1/PD-L1 pathway, promotes CD8+ T cell apoptosis, and facilitates immune escape in NSCLC.
Confidence score:

0.8322
Other information
Title:

Immunomodulatory functions of the circ_001678/miRNA-326/ZEB1 axis in non-small cell lung cancer via the regulation of PD-1/PD-L1 pathway.

Journal: Human molecular genetics
Published: 2022
PubMed ID: 35848890
Study type:

combined biological and clinical study
Data availability: available from the corresponding author on reasonable request
Code availability: -