| Expression pattern: |
UP |
| Associated gene: |
HSPA8 |
| Associated microRNA: |
miR-26a-5p |
| Biological function: |
Promotes proliferation, colony formation, invasion and migration; facilitates G1/S transition (reduces G0/G1 arrest) in breast cancer cells. |
| Molecular mechanism: |
Acts as a ceRNA/miRNA sponge: circDENND4C competitively binds miR-26a-5p to relieve repression of HSPA8. |
| Biological pathway or process: |
proliferation (promotes); cell cycle (promotes); migration (promotes); invasion (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Clinical Sample Validation; Actinomycin D / DRB Stability Assay; Transfection; MTT; Colony Formation Assay; Cell Cycle Assay; Transwell Assay; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); Survival Analysis; Bioinformatics Analysis; Western Blot |
| Clinical significance: |
High circDENND4C expression is associated with poor prognosis and lower 5-year overall survival in BC patients. |
| Description: |
circDENND4C is upregulated in breast cancer and functions as an oncogenic circRNA. It sponges miR-26a-5p to increase HSPA8, thereby promoting proliferation, cell-cycle progression, migration and invasion, and its high expression correlates with worse survival. |
| Confidence score: |
0.8001 |