| Expression pattern: |
UP |
| Associated gene: |
IVNS1ABP, Ago2 |
| Associated microRNA: |
miR-145-5p |
| Biological function: |
promotes cell viability/proliferation, colony formation, invasion, and in vivo tumor growth in gastric cancer |
| Molecular mechanism: |
Acts as a miRNA sponge for miR-145-5p, affecting IVNS1ABP expression (ceRNA-like mechanism) and associating with Ago2 complex. |
| Biological pathway or process: |
ceRNA regulation (promotes); proliferation (promotes); invasion (promotes) |
| Detected method: |
Q
H
M
|
| Validation methods: |
Microarray; RT-qPCR; FISH / smFISH; RNase R Treatment; Actinomycin D / DRB Stability Assay; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); Transfection; MTT; Colony Formation Assay; Transwell Assay; Western Blot; In Vivo Animal Model; Clinical Sample Validation; Survival Analysis; Bioinformatics Analysis; ROC Analysis |
| Clinical significance: |
High circDUSP16 expression is an independent prognostic factor of poor survival in patients with GC. |
| Description: |
circDUSP16 (hsa_circ_0003855), derived from DUSP16, is up-regulated in gastric cancer tissues and primarily localized in the cytoplasm. It promotes GC cell proliferation/viability, colony formation, invasion, and xenograft tumor growth by sponging miR-145-5p, thereby relieving repression of IVNS1ABP. High circDUSP16 expression predicts poorer overall survival and is an independent prognostic factor in GC. |
| Confidence score: |
0.8655 |