| Expression pattern: |
DN |
| Associated gene: |
RFX7 |
| Associated microRNA: |
miR-616-3p |
| Biological function: |
Overexpression of circ_0030042 facilitated endothelial cell viability and proliferation, repressed apoptosis, and reduced inflammatory factor levels in ox-LDL-induced HUVECs and HAECs, suggesting a protective role against atherosclerotic endothelial cell injury. |
| Molecular mechanism: |
circ_0030042 acts as a sponge for miR-616-3p and increases RFX7 expression, thereby regulating proliferation, apoptosis, and inflammatory factor content in ox-LDL-induced endothelial cells. |
| Biological pathway or process: |
proliferation (promotes); apoptosis (inhibits); inflammation (inhibits); ceRNA regulation (other) |
| Detected method: |
Q
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; RT-qPCR; Bioinformatics Analysis; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); RNA Pull-Down; Transfection; CCK8; EdU Staining; Annexin V/PI Flow Cytometry; ELISA; Western Blot |
| Clinical significance: |
Circ_0030042 is likely to be a potential biomarker for the future treatment of patients with AS. |
| Description: |
This study identifies hsa_circ_0030042 as a down-regulated circRNA in ox-LDL-induced endothelial cell models of atherosclerosis. Functionally, circ_0030042 protects endothelial cells by promoting proliferation while suppressing apoptosis and inflammatory cytokine release. Mechanistically, it acts through the circ_0030042/miR-616-3p/RFX7 regulatory network and may represent a potential biomarker or therapeutic target for AS. |
| Confidence score: |
0.7817 |