circRNA basic information
circBase ID: -
Name: hsa_circ_RNF111
Synonym: circ-RNF111 / CircRNA ring finger protein 111
Host Gene: RNF111
Genomic location(hg19): -
Genomic location(hg38): -
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0003175
MONDO name: salivary gland adenoid cystic carcinoma
Disease details: salivary adenoid cystic carcinoma / SACC
Disease DO ID:
 4866
Disease MeSH ID:
 -
Disease NCIt ID:
 C8026
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

SACC specimens; normal salivary gland (NSG) specimens; tumor tissues; lung tissue
Cell lines:

SACC-LM; SACC-83; HSG; BV2
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: HMGB2
Associated microRNA: miR-361-5p
Biological function: circ-RNF111 promotes growth, viability, colony formation, migration, invasion, perineural invasion, lung tumor burden, and poor clinical behavior in SACC; circ-RNF111 depletion increases apoptosis and suppresses growth and PNI.
Molecular mechanism: circ-RNF111 acts as a miRNA sponge for miR-361-5p and positively regulates HMGB2 expression, thereby inducing SACC-LM cell development and perineural invasion.
Biological pathway or process:

ceRNA regulation (promotes); proliferation (promotes); apoptosis (inhibits); migration (promotes); invasion (promotes); metastasis (promotes); other pathway/process (promotes)
Detected method:
Q
Validation methods:

RNase R Treatment; Actinomycin D / DRB Stability Assay; RT-qPCR; Clinical Sample Validation; Nuclear-Cytoplasmic Fractionation; Transfection; CCK8; Colony Formation Assay; Annexin V/PI Flow Cytometry; Wound Healing Assay; Transwell Assay; Luciferase Reporter Assay; RNA Pull-Down; Western Blot; In Vivo Animal Model; H&E Staining; Survival Analysis; ROC Analysis; Bioinformatics Analysis; Cohort Study
Clinical significance:

High circ-RNF111 expression was associated with tumor site, clinical stage, surgical margin, local recurrence, distant metastasis, histological subtype, poor overall survival, poor distant metastasis-free survival, poor recurrence-free survival, and diagnostic value for SACC.
Description:

circ-RNF111 is up-regulated in SACC specimens and SACC cell lines and is mainly cytoplasmic. It promotes SACC-LM cell growth, migration, invasion, and perineural invasion by sponging miR-361-5p and increasing HMGB2 expression. High circ-RNF111 expression is associated with adverse clinicopathological features, poor survival, and diagnostic potential in SACC.
Confidence score:

0.8659
Other information
Title:

Induction of perineural invasion in salivary adenoid cystic carcinoma by circular RNA RNF111.

Journal: Clinical & translational oncology : official publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico
Published: 2023
PubMed ID: 37222950
Study type:

combined biological and clinical study
Data availability: -
Code availability: -