circRNA basic information
circBase ID: hsa_circ_0043278
Name: hsa_circ_TADA2A
Synonym: -
Host Gene: TADA2A
Genomic location(hg19): chr17:35797838-35800763:+
Genomic location(hg38): chr17:37437737-37440662:+
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0009807
MONDO name: osteosarcoma
Disease details: osteosarcoma
Disease DO ID:
 3347
Disease MeSH ID:
 D012516
Disease NCIt ID:
 C9145
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

OS tissue; chondroma tissue
Cell lines:

HOS; 143B; MG-63; U2OS; SJSA-1; hFOB1.19; HEK-293
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: CREB3, AGO2
Associated microRNA: miR-203a-3p
Biological function: promotes migration, invasion, proliferation, tumorigenesis and metastasis; inhibits apoptosis
Molecular mechanism: ceRNA/miRNA sponge mechanism: circTADA2A sponges miR-203a-3p to upregulate CREB3 expression
Biological pathway or process:

ceRNA regulation (promotes); migration (promotes); invasion (promotes); proliferation (promotes); apoptosis (inhibits); metastasis (promotes); EMT (promotes)
Detected method:
Q
H
M
Validation methods:

Microarray; RT-qPCR; FISH / smFISH; Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; Transfection; Transwell Assay; Wound Healing Assay; Colony Formation Assay; CCK8; Annexin V/PI Flow Cytometry; Western Blot; In Vivo Animal Model; IHC (Immunohistochemistry); TUNEL
Clinical significance:

-
Description:

circTADA2A (hsa_circ_0043278), derived from TADA2A exons 5 and 6, is up-regulated in osteosarcoma tissues and cell lines and mainly localizes in the cytoplasm. It promotes osteosarcoma cell proliferation, migration, invasion, tumorigenesis and metastasis while suppressing apoptosis by acting as a miR-203a-3p sponge to increase CREB3 expression (circTADA2A/miR-203a-3p/CREB3 axis).
Confidence score:

0.8379
Other information
Title:

Circular RNA circTADA2A promotes osteosarcoma progression and metastasis by sponging miR-203a-3p and regulating CREB3 expression.

Journal: Molecular cancer
Published: 2019
PubMed ID: 30940151
Study type:

combined biological and clinical study
Data availability: GSE96964
Code availability: -