circRNA basic information
circBase ID: hsa_circ_0061825
Name: hsa_circ_TFF1
Synonym: circ-TFF1
Host Gene: TFF1
Genomic location(hg19): chr21:43782390-43786644:-
Genomic location(hg38): chr21:42362281-42366535:-
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0007254
MONDO name: breast cancer
Disease details: Breast cancer
Disease DO ID:
 1612
Disease MeSH ID:
 -
Disease NCIt ID:
 C9335
Disease ICD11 ID:
 1047754165
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

BC tumor specimens; para-hepatic normal breast tissue; tumor tissue; normal tissue; nude mouse tumor tissue
Cell lines:

MCF-10A; MDA-MB-361; SK-Br-3; HUVEC
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: IRAK1, Ago2, VEGFA, VE-cadherin, KI67
Associated microRNA: miR-129-2-3p
Biological function: Promotes breast cancer cell proliferation, migration, invasion, tube formation/angiogenesis, and tumor growth; inhibits apoptosis.
Molecular mechanism: circ-TFF1 functions as a ceRNA that sponges miR-129-2-3p to regulate IRAK1 expression, thereby promoting breast cancer progression.
Biological pathway or process:

ceRNA regulation (promotes); proliferation (promotes); migration (promotes); invasion (promotes); angiogenesis (promotes); apoptosis (inhibits); metastasis (promotes)
Detected method:
Q
Validation methods:

RT-qPCR; RNase R Treatment; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; Bioinformatics Analysis; Transfection; MTT; EdU Staining; Colony Formation Assay; Transwell Assay; Annexin V/PI Flow Cytometry; Tube Formation Assay; Western Blot; IHC (Immunohistochemistry); In Vivo Animal Model
Clinical significance:

High circ-TFF1 expression was correlated with tumor size, lymph node metastasis, and TNM stage in breast cancer patients.
Description:

circ-TFF1 is up-regulated in breast cancer tissues and cell lines and is mainly cytoplasmic. It promotes malignant breast cancer phenotypes, including proliferation, migration, invasion, angiogenesis/tube formation, and xenograft tumor growth, while suppressing apoptosis. Mechanistically, circ-TFF1 sponges miR-129-2-3p to up-regulate IRAK1, forming a circ-TFF1/miR-129-2-3p/IRAK1 regulatory axis.
Confidence score:

0.8175
Other information
Title:

Circ-TFF1 Promotes Breast Cancer Progression Through the miR-129-2-3p/IRAK1 Axis.

Journal: Biochemical genetics
Published: 2022
PubMed ID: 35279761
Study type:

combined biological and clinical study
Data availability: -
Code availability: -