| Expression pattern: |
UP |
| Associated gene: |
TPR |
| Associated microRNA: |
miR-1200 |
| Biological function: |
Promotes TNBC cell proliferation, migration, invasion and EMT; promotes xenograft tumor growth; decreases sensitivity to pirarubicin (THP). |
| Molecular mechanism: |
Acts as a ceRNA/miRNA sponge for miR-1200 to regulate TPR, thereby activating the RAS-RAF-MEK-ERK pathway and promoting malignant progression; contributes to THP (pirarubicin) resistance/sensitivity changes. |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); EMT (promotes); ERK (promotes); drug resistance (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
S
M
|
| Validation methods: |
RNA-seq; Microarray; RT-qPCR; Back-Splice Junction PCR / divergent primers PCR; Sanger Sequencing; Nuclear-Cytoplasmic Fractionation; RNase R Treatment; Actinomycin D / DRB Stability Assay; Luciferase Reporter Assay; RNA Pull-Down; Transfection; CCK8; EdU Staining; Transwell Assay; Wound Healing Assay; Western Blot; In Vivo Animal Model; ROC Analysis; Bioinformatics Analysis; Clinical Sample Validation |
| Clinical significance: |
Upregulated in TNBC tumor tissues and plasma exosomes; ROC AUC 0.787 for distinguishing BC from adjacent normal tissue; high expression associated with lymph node metastasis, vascular infiltration, and (in plasma exosomes) tumor size; proposed prognostic biomarker. |
| Description: |
CircZCCHC2 (hsa_circ_0000854, from ZCCHC2) is upregulated in TNBC tissues, TNBC cell lines, and patient plasma exosomes. It promotes proliferation, migration, invasion and EMT and enhances xenograft tumor growth. Mechanistically, it primarily localizes in the cytoplasm and sponges miR-1200 to upregulate TPR, activating the RAS-RAF-MEK-ERK pathway and decreasing sensitivity to pirarubicin (THP). |
| Confidence score: |
0.8709 |