| Expression pattern: |
UP |
| Associated gene: |
COL11A1 |
| Associated microRNA: |
miR-653-5p |
| Biological function: |
Promotes NSCLC cell proliferation, migration, invasion, EMT, metastasis-related malignant behaviors, and tumor growth in vivo. |
| Molecular mechanism: |
circRANGAP1 functions as a ceRNA/miRNA sponge for miR-653-5p, relieving miR-653-5p-mediated repression of COL11A1 and thereby increasing COL11A1 expression. |
| Biological pathway or process: |
ceRNA regulation (promotes); proliferation (promotes); migration (promotes); invasion (promotes); metastasis (promotes); EMT (promotes) |
| Detected method: |
Q
M
|
| Validation methods: |
RT-qPCR; Bioinformatics Analysis; Clinical Sample Validation; Survival Analysis; Nuclear-Cytoplasmic Fractionation; Transfection; EdU Staining; Colony Formation Assay; Wound Healing Assay; Transwell Assay; Western Blot; RNA Pull-Down; Luciferase Reporter Assay; In Vivo Animal Model; IHC (Immunohistochemistry) |
| Clinical significance: |
High circRANGAP1 expression is associated with poorer survival in NSCLC patients and may serve as an underlying diagnostic and therapeutic agent. |
| Description: |
circRANGAP1 is up-regulated in NSCLC tissues and cell lines, and high expression is associated with poorer patient survival. Functionally, circRANGAP1 promotes NSCLC proliferation, migration, invasion, EMT, and xenograft tumor growth. Mechanistically, it acts as a miR-653-5p sponge to increase COL11A1 expression through a circRANGAP1/miR-653-5p/COL11A1 ceRNA axis. |
| Confidence score: |
0.798 |