| Expression pattern: |
UP |
| Associated gene: |
NUCB2 protein, JAK1, STAT3 |
| Associated microRNA: |
miR-338-3p |
| Biological function: |
Promotes RCC cell viability, colony formation, migration, invasion, angiogenesis and tumor formation; inhibits apoptosis. |
| Molecular mechanism: |
circ_0000274 acts as a ceRNA by sponging miR-338-3p to positively regulate NUCB2 protein and activates the JAK1/STAT3 protein signaling pathway. |
| Biological pathway or process: |
JAK/STAT (promotes); ceRNA regulation (promotes); proliferation (promotes); migration (promotes); invasion (promotes); angiogenesis (promotes); apoptosis (inhibits); metastasis (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RNase R Treatment; RT-qPCR; Clinical Sample Validation; Transfection; CCK8; Colony Formation Assay; Transwell Assay; Tube Formation Assay; Annexin V/PI Flow Cytometry; Western Blot; Bioinformatics Analysis; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); In Vivo Animal Model; IHC (Immunohistochemistry) |
| Clinical significance: |
High circ_0000274 expression was linked to advanced TNM stage and metastasis in RCC; it might provide a promising target for RCC treatment. |
| Description: |
circ_0000274 is up-regulated in RCC tissues and cells and acts as an oncogenic circRNA. It promotes RCC growth, migration, invasion, angiogenesis and in vivo tumor formation while suppressing apoptosis through the miR-338-3p/NUCB2 axis and activation of JAK1/STAT3 signaling. Its higher expression is associated with advanced TNM stage and metastasis. |
| Confidence score: |
0.8006 |