circRNA basic information
circBase ID: hsa_circ_0003645
Name: -
Synonym: circ_0003645
Host Gene: -
Genomic location(hg19): chr16:19656207-19663412:+
Genomic location(hg38): chr16:19644885-19652090:+
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0005233
MONDO name: non-small cell lung carcinoma
Disease details: non-small cell lung cancer / NSCLC
Disease DO ID:
 3908
Disease MeSH ID:
 D002289
Disease NCIt ID:
 C2926
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

NSCLC tissue specimens; matched normal tissues; cancerous tissues; paired non-cancerous tissue samples
Cell lines:

16HBE; H358; H1299; H1581; A549
In vivo animal model:

-
circRNA-disease information
Expression pattern:
UP
Associated gene: TMEM14A
Associated microRNA: miR-1179
Biological function: circ_0003645 functions as an oncogene in NSCLC cells, promoting cell proliferation, viability, migration and invasion, and inhibiting apoptosis.
Molecular mechanism: circ_0003645 acts as a ceRNA to sponge miR-1179, leading to increased TMEM14A expression and facilitated NSCLC cell progression.
Biological pathway or process:

ceRNA regulation (promotes); proliferation (promotes); apoptosis (inhibits); migration (promotes); invasion (promotes)
Detected method:
Q
Validation methods:

RT-qPCR; Clinical Sample Validation; Transfection; CCK8; Colony Formation Assay; Annexin V/PI Flow Cytometry; Transwell Assay; Luciferase Reporter Assay; Western Blot; Cohort Study; Survival Analysis; Bioinformatics Analysis
Clinical significance:

High circ_0003645 expression is associated with advanced TNM stages, positive lymph node invasion, worse overall survival, and independent prognostic value in NSCLC patients.
Description:

circ_0003645 is up-regulated in NSCLC tissues and cell lines, and high expression predicts unfavorable prognosis. Functionally, it acts as an oncogenic circRNA that promotes proliferation, migration and invasion while inhibiting apoptosis. Mechanistically, circ_0003645 sponges miR-1179 to elevate TMEM14A expression, forming a circ_0003645/miR-1179/TMEM14A regulatory axis.
Confidence score:

0.7408
Other information
Title:

Elevation of circular RNA circ_0003645 forecasts unfavorable prognosis and facilitates cell progression via miR-1179/TMEM14A pathway in non-small cell lung cancer.

Journal: Biochemical and biophysical research communications
Published: 2019
PubMed ID: 30853176
Study type:

combined biological and clinical study
Data availability: The datasets supporting the findings of this study are included in the article.
Code availability: -