| Expression pattern: |
DN |
| Associated gene: |
SND1, Tudor domain of SND1, M6A modified RNA, c-myb, STAT6, c-myb mRNA, c-myb CRD |
| Associated microRNA: |
- |
| Biological function: |
Inhibits proliferation and invasion of osteosarcoma cells and partially reverses the SND1-promoted proliferation and invasiveness of osteosarcoma cells. |
| Molecular mechanism: |
circ0024831 directly binds the Tudor domain of SND1 in the cytoplasm, blocks SND1 recognition of M6A modified RNA, reduces downstream target mRNA stability, and inhibits downstream regulatory protein expression. |
| Biological pathway or process: |
proliferation (inhibits); invasion (inhibits); mRNA stability (inhibits); m6A modification (other); other pathway/process (other) |
| Detected method: |
Q
S
|
| Validation methods: |
RT-qPCR; Bioinformatics Analysis; RIP (RNA Immunoprecipitation); FISH / smFISH; Transfection; Transwell Assay; MTT; Annexin V/PI Flow Cytometry; Luciferase Reporter Assay; Western Blot; Clinical Sample Validation |
| Clinical significance: |
May provide a new theoretical basis for revealing the mechanism for occurrence and development of osteosarcoma for targeted therapy; down-regulation of circ0024831 promotes osteosarcoma occurrence and development. |
| Description: |
circ0024831 is down-regulated in osteosarcoma and acts as a suppressive circRNA. It binds SND1 in the cytoplasm through the Tudor-domain-related interaction, interferes with SND1 recognition of M6A-modified RNA, decreases downstream mRNA stability and c-myb/STAT6 expression, and inhibits osteosarcoma cell proliferation and invasion. |
| Confidence score: |
0.7283 |