| Expression pattern: |
UP |
| Associated gene: |
NFIB |
| Associated microRNA: |
miR-146b-5p |
| Biological function: |
circ_VCAN promotes glioma cell proliferation, migration and invasion, suppresses apoptosis, and attenuates the inhibitory effect of sevoflurane on glioma tumor growth. |
| Molecular mechanism: |
circ_VCAN acts as a competing endogenous RNA sponge for miR-146b-5p, thereby upregulating NFIB expression and mediating sevoflurane-related inhibition of glioma malignant phenotypes. |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); apoptosis (inhibits); ceRNA regulation (promotes); other pathway/process (other) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Actinomycin D / DRB Stability Assay; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; Luciferase Reporter Assay; Transfection; CCK8; MTT; EdU Staining; Colony Formation Assay; Annexin V/PI Flow Cytometry; Transwell Assay; Western Blot; IHC (Immunohistochemistry); In Vivo Animal Model; Bioinformatics Analysis |
| Clinical significance: |
circ_VCAN was apparently upregulated in glioma tissues relative to normal tissues, suggesting potential disease relevance, but no diagnostic or prognostic performance was reported. |
| Description: |
circ_VCAN is upregulated in glioma tissues and is downregulated by sevoflurane treatment in glioma cells. Functionally, circ_VCAN overexpression counteracts sevoflurane-mediated inhibition of proliferation, migration, invasion and tumor growth, while reducing sevoflurane-induced apoptosis. Mechanistically, circ_VCAN sponges miR-146b-5p to upregulate NFIB, forming a circ_VCAN/miR-146b-5p/NFIB axis in glioma. |
| Confidence score: |
0.7861 |