| Expression pattern: |
UP |
| Associated gene: |
FOXO1, Ago2, P65, NF-kB pathway |
| Associated microRNA: |
miR-514a-3p |
| Biological function: |
Facilitates TNF-alpha-induced VSMC proliferation and migration and inhibits apoptosis; contributes to AS progression. |
| Molecular mechanism: |
hsa_circ_0030042 acts as a ceRNA by sponging miR-514a-3p to positively regulate FOXO1 expression and also modulates the NF-kB pathway through P65 translocation. |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); apoptosis (inhibits); ceRNA regulation (promotes); NF-kappaB (other) |
| Detected method: |
Q
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; RT-qPCR; Nuclear-Cytoplasmic Fractionation; FISH / smFISH; IF (Immunofluorescence); RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; Transfection; CCK8; EdU Staining; TUNEL; Annexin V/PI Flow Cytometry; Wound Healing Assay; Transwell Assay; Western Blot; Bioinformatics Analysis |
| Clinical significance: |
Potential target for AS treatment. |
| Description: |
hsa_circ_0030042 is up-regulated in TNF-alpha- or IL-6-induced human VSMCs used as an atherosclerosis cell model. It promotes VSMC proliferation and migration while inhibiting apoptosis, mainly through sponging miR-514a-3p to up-regulate FOXO1 and additionally modulating the NF-kB pathway via P65 translocation. |
| Confidence score: |
0.8095 |