| Expression pattern: |
UP |
| Associated gene: |
TNFSF11 |
| Associated microRNA: |
miR-149-5p |
| Biological function: |
circ_CDYL promotes Ang II-induced podocyte apoptosis; knockdown of circ_CDYL inhibits apoptosis in podocytes and protects against Ang II-induced injury in the MN model. |
| Molecular mechanism: |
circ_CDYL functions through a ceRNA-like mechanism by interacting with miR-149-5p and modulating TNFSF11 expression, thereby regulating Ang II-induced podocyte apoptosis. |
| Biological pathway or process: |
apoptosis (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; Clinical Sample Validation; Transfection; Annexin V/PI Flow Cytometry; Luciferase Reporter Assay; Western Blot; ROC Analysis; Bioinformatics Analysis |
| Clinical significance: |
Serum circ_CDYL may serve as a new non-invasive and specific marker for MN patients, with an AUC of 0.8600. |
| Description: |
circ_CDYL is up-regulated in renal tissues and serum from MN patients and in Ang II-induced human podocytes. Functionally, circ_CDYL promotes podocyte apoptosis, while circ_CDYL knockdown protects against Ang II-induced apoptosis. Mechanistically, circ_CDYL interacts with miR-149-5p to regulate TNFSF11 expression, and serum circ_CDYL shows potential diagnostic value for MN. |
| Confidence score: |
0.7905 |