| Expression pattern: |
UP |
| Associated gene: |
ADAMTS5 |
| Associated microRNA: |
miR-502-5p |
| Biological function: |
circ-PRKCH contributes to IL-1b-treated chondrocyte phenotypic changes; circ-PRKCH knockdown promotes cell proliferation and migration and inhibits apoptosis and inflammatory response. |
| Molecular mechanism: |
circ-PRKCH acts as a ceRNA by sponging miR-502-5p to elevate ADAMTS5 expression; exosomes derived from IL-1b-treated chondrocytes transfer circ-PRKCH across cells. |
| Biological pathway or process: |
ceRNA regulation (promotes); proliferation (inhibits); migration (inhibits); apoptosis (promotes); inflammation (promotes); other pathway/process (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RNase R Treatment; RT-qPCR; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; Transfection; CCK8; EdU Staining; Annexin V/PI Flow Cytometry; Wound Healing Assay; ELISA; Western Blot; Bioinformatics Analysis |
| Clinical significance: |
circ-PRKCH might provide a promising biomarker for OA treatment. |
| Description: |
circ-PRKCH is up-regulated in OA cartilage, OA serum exosomes, and IL-1beta-treated human chondrocytes. It promotes OA-like chondrocyte phenotypic injury through a ceRNA mechanism in which circ-PRKCH sponges miR-502-5p to increase ADAMTS5 expression, while circ-PRKCH knockdown alleviates apoptosis and inflammation and restores proliferation and migration. The study also suggests that exosomes can mediate intercellular transfer of circ-PRKCH. |
| Confidence score: |
0.7799 |