| Expression pattern: |
DS |
| Associated gene: |
TGFBR3 |
| Associated microRNA: |
miR-513a-5p |
| Biological function: |
Protects endothelial cells from injury; promotes endothelial cell proliferation and viability; inhibits apoptosis and migration; delays atherogenesis and reduces intraplaque neovascularization. |
| Molecular mechanism: |
Acts as a ceRNA by sponging miR-513a-5p to upregulate TGFBR3. |
| Biological pathway or process: |
ceRNA regulation (other); proliferation (promotes); apoptosis (inhibits); migration (inhibits); angiogenesis (inhibits) |
| Detected method: |
Q
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; RT-qPCR; Clinical Sample Validation; Bioinformatics Analysis; Luciferase Reporter Assay; Transfection; CCK8; EdU Staining; Wound Healing Assay; In Vivo Animal Model; H&E Staining; IHC (Immunohistochemistry) |
| Clinical significance: |
Proposed as a biomarker for atherogenesis; lower circ_0001785 levels were associated with more severe coronary stenosis. |
| Description: |
Exosome-derived circ_0001785 is dysregulated in CHD/atherosclerosis (reduced in circulating blood/leukocytes but increased in plaque tissue). Functionally, circ_0001785 protects endothelial cells and delays atherogenesis by acting as a ceRNA that sponges miR-513a-5p, thereby upregulating TGFBR3 to promote proliferation and inhibit apoptosis/migration and intraplaque neovascularization. |
| Confidence score: |
0.8166 |